Tests and Services Manual: Enteric Bacteriology

CAMPYLOBACTER ID

Specimen:

Isolate

Method:

Conventional biochemicals

Turn Around Time:

48 – 96 hrs

Special Requirements:

RT on Campy BA media
ENTERIC PATHOGEN
PROFILE (Salmonella, Shigella,
Yersinia, Campylobacter,
Aeromonas, Pleisomonas,
Enterohaemorrhagic
Escherichia coli),
Bacillus cereus,
Clostridium perfringens

Specimen:

Stool

Method:

Culture; if positive or suspicious, conventional biochemicals and serotyping performed

Turn Around Time:

3 – 10 days

Special Requirements:

If stool, send in transport media (Cairy Blair) at Room Temperature. For DOHMH only.
ID OF MISCELLANEOUS
ENTEROBACTERIACEAE
(Isolates that the
referring laboratory
does not consider
to be either Salmonella
nor Shigella)

Specimen:

Isolate

Method:

Conventional biochemicals and serotyping

Turn Around Time:

3 – 10 days

Special Requirements:

Send at Room Temperature
SALMONELLA ID
SEROTYPING
(DOH Referrals Only)

Specimen:

Isolate

Method:

Conventional biochemicals (ID). PCR and serology (serotyping)

Turn Around Time:

3 – 10 days

Special Requirements:

Send at Room Temperature. DOHMH only
SHIGELLA SPP.,
ID, SEROTYPING,
and AST

Specimen:

Isolate

Method:

Conventional biochemicals serotyping, and AST

Turn Around Time:

3 – 10 days

Special Requirements:

Send at Room Temperature
STX E. coli R/O

Specimen:

Stool or broth

Method:

Culture; Assay for Shiga toxin I & II performed if positive, ID and Serotyping

Turn Around Time:

48 – 96 hrs

Special Requirements:

Send stool and GN broth in cold pack
STX E. coli
Confirmation

Specimen:

Reflexive test, performed when E. coli/other EHEC is identified.

Method:

EIA and serotyping

Turn Around Time:

48 – 96 hrs

Special Requirements:

Isolate; send at Room Temperature
VIBRIO CHOLERAE/
Vibrio spp.

Specimen:

Isolate

Method:

Conventional biochemicals and serotyping

Turn Around Time:

3 – 10 days

Special Requirements:

Send at Room Temperature
Yersinia spp.

Specimen:

Isolate

Method:

Conventional biochemical

Turn Around Time:

3 – 10 days

Special Requirements:

Isolate; send at Room temperature