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Department of Forensic Biology

Forensic Biology Protocols for Mitochondrial DNA Analysis Manuals

This manual contains individual procedures pertaining to Mitochondrial DNA testing of biological specimens.

Procedures on this page are effective as of June 20, 2016 and later. The dates effective are shown as MM/DD/YY - MM/DD/YY in the file name.

Note: The most current manual in each section is the first link listed.

Click or press the enter key on any topic below to access the relevant PDF file of the associated procedure.

Mitochondrial DNA Guidelines

Hair Evidence Examination

Washing Hair for Mitochondrial or Nuclear DNA Testing

Organic Extraction for Mitochondrial or Nuclear DNA Testing

Duplex Mitochondrial DNA PCR Amplification

Quantitation using Agilent 2100 Bioanalyzer

Cycle-Sequencing

As of 8/23/21 the Cycle – Sequencing procedure was incorporated into the combined procedure named “Mito Cycle Sequencing, Cleanup, and 3130xl Sequencing".

  • Cycle-Sequencing effective 12/13/19 - 08/22/21
  • Cycle-Sequencing effective 09/14/17 - 12/12/19
  • Cycle-Sequencing effective 06/20/16 - 09/13/17
  • SDS Cleanup

    As of 8/23/21 the SDS Cleanup procedure was incorporated into the combined procedure named “Mito Cycle Sequencing, Cleanup, and 3130xl Sequencing”.

  • SDS Cleanup effective 09/15/17 - 08/22-21
  • SDS Cleanup effective 06/20/16 - 09/14/17
  • ExoSAP-IT Sample Cleanup

    Centri-Sep Sample Filtration

    As of 8/23/21 the Centri-Sep Sample Filtration procedure was incorporated into the combined procedure named “Mito Cycle Sequencing, Cleanup, and 3130xl Sequencing”.

  • Centri-Sep Sample Filtration effective 09/14/17 - 08/22/21
  • Centri-Sep Sample Filtration effective 06/20/16 - 09/13/17
  • ABI 3130xl Sequencing

    As of 8/23/21 the ABI 3130xl Sequencing procedure was incorporated into the combined procedure named “Mito Cycle Sequencing, Cleanup, and 3130xl Sequencing”.

  • ABI 3130xl Sequencing effective 09/15/17 - 08/22/21
  • ABI 3130xl Sequencing effective 06/20/16 - 09/14/17
  • Mito Cycle Sequencing, Cleanup, and 3130xl Sequencing

    Mitochondrial DNA Sequencing Analysis

    Editing Guidelines

    Sequence Nomenclature and Alignment

    Interpretation Guidelines

    Statistical Analysis

    Creation of a Casefile CD

    References

    Preparation of Phase Lock Gel (PLG) Tubes

    This procedure has been retired as of 07/09/19.

  • Preparation of Phase Lock Gel (PLG) Tubes effective 09/15/17
  • Appendix A - Oligonucleotide Primer Sequences

    Appendix B - Mitochondrial DNA Primer Locations

    Appendix C - Revised Cambridge Reference Sequence